Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: The validation of recombinant adenovirus BMP9 and siBMP9. ( A ) The expression of BMP9 in normal bladder mucosa ( n = 126), superficial bladder cancer ( n = 68), and infiltrating bladder cancer ( n = 62) in the Lee Bladder database. p = 0.007; ( B ) The expression levels of BMP9 in T24 and BIU-87 cells were detected by western blot; ( C ) The BMP9 was up-regulated in BIU-87 cells after being transfected with AdBMP9 compared to the control group; ( D ) The BMP9 was down-regulated in T24 cells after being transfected with AdsiBMP9 compared to the control group. Data are shown as mean ± SD. ** p < 0.01.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Recombinant, Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: BMP9 up-regulated the expression of lncRNA UCA1 in bladder cancer cells. ( A ) Five common lncRNA were screened in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( B ) The expression of lncRNA UCA1 were verified in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( C ) The expression of lncRNA UCA1 were tested in T24 cells after being transfected with AdsiBMP9 by RT-PCR; ( D ) The inhibitory effect of siUCA1 were analyzed by RT-PCR in BIU-87 cells after being co-transfected with AdBMP9 and siUCA1. Data are shown as mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. control groups.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Activation of the NLRP3 Inflammasome Pathway by Uropathogenic Escherichia coli Is Virulence Factor-Dependent and Influences Colonization of Bladder Epithelial Cells

doi: 10.3389/fcimb.2018.00081

Figure Lengend Snippet: The importance of different virulence factors for IL-1β release and caspase-1 activity. The bladder epithelial cell line 5637 (A–D) and a spontaneously transformed bladder epithelial cell line HBLAK (E) were infected with CFT073, CFT073Δpap, CFT073ΔfimH, CFT073ΔhlyA, CFT073ΔhlyA/pGNH404 and CFT073 fim L-ON at MOI 10 for 3 (A,C) and 6 h (B,D,E) . IL-1β release (A,B,E) and caspase-1 activity (C,D) were measured. Caspase-1 results are presented as fold increase of mean fluorescence units (MFU) compared to unstimulated control cells. Hemolysin activity on blood agar was evaluated for CFT073, CFT073 fim L-ON, and CFT073ΔhlyA after overnight incubation (F) . Data are presented as mean ± SEM ( n = 3 independent experiments). Asterisks denote statistical significance compared to respective unstimulated control (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: The human bladder epithelial cell line HBLAK (CELLnTEC Advanced Cell Systems AG, Bern, Switzerland) has been isolated from a healthy bladder and been spontaneously transformed providing the convenience of long-term cell growth without senescence.

Techniques: Activity Assay, Transformation Assay, Infection, Fluorescence, Incubation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: AKR1C2 protein expression in HT1376-CisR cells was markedly increased in comparison with the parental cells. AKR1C2 small interfering RNA reduced expression by ~80% in HT1376-CisR cells. AKR1C2 and β-tubulin exhibit discrete bands of the same molecular weight (AKR1C2, 37 kDa; β-tubulin, 51 kDa). AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Expressing, Comparison, Small Interfering RNA, Molecular Weight

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Effect of AKR1C2 expression on cisplatin IC 50 values in parental and HT1376-CisR cells. Cells were treated with various cisplatin concentrations for 72 h, and then quantified using a cell counter. Each assay was performed in triplicate. Cell survival in the absence of cisplatin was set as 100%. (A) Silencing AKR1C2 restored HT1376-CisR cell response to cisplatin. (B) Inhibition of AKR1C2 by 100 μM 5β-cholanic acid restored the HT1376-CisR response to cisplatin. * P<0.05, vs. HT1376-CisR. Bars indicate standard deviation. AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Expressing, Inhibition, Standard Deviation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Effect of cisplatin on intracellular ROS in HT1376 cells. Exposure to cisplatin increased the levels of intracellular ROS in HT1376 cells in a dose-dependent manner. * P<0.05, vs. HT1376 cells cultured without cisplatin. Bars indicate standard deviation. ROS, reactive oxygen species.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Cell Culture, Standard Deviation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Relative values of intracellular ROS measured using a 2,7-dichlorodihydrofluorescein diacetate probe. (A) Basal intracellular ROS levels in HT1376, HT1376-CisR and HT1376-CisR cells transiently transfected with AKR1C2 small interfering RNA [HT1376-CisR-AKR1C2(−)]. * P<0.05 and $ P<0.05, vs. HT1376 and HT1376-CisR cells cultured without cisplatin, respectively. (B) Effect of 10 −4 M cisplatin exposure on intracellular ROS in these cells. (C) Effect of 5 μM menadione on intracellular ROS in these cells. * P<0.05 vs. control cells cultured without cisplatin or menadione. Bars indicate standard deviation. AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant; ROS, reactive oxygen species.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Transfection, Small Interfering RNA, Cell Culture, Control, Standard Deviation

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: The expression of microRNAs in the cell lines. The expression of miR-182, miR-183, and miR-96 increased, whereas the expression of miR-145, miR-1, miR-133a, miR-99a, miR-125b, miR-195, miR-100, and miR-29c decreased in bladder tumor cell lines compared to normal human urothelial tissue. (RQ, relative quantitation).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNAs associated with proliferative activity. A. The 5637 and T24 urothelial cancer cell lines revealed increased proliferative activity than 253J, HT1376, and RT4 cell lines in proliferation assay. B. The expression of miR-133a and miR-145 decreased as the proliferation increased. (RQ, relative quantitation).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Activity Assay, Proliferation Assay, Expressing, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNAs expression in formalin-fixed and paraffin-embedded tissue of urothelial neoplasms. The expression of miR-145, miR-133a and miR-205 in formalin-fixed paraffin-embedded tissue. When whole section including stromal tissue was used (stroma-rich), the expression of miR-145 increased in invasive urothelial carcinoma. When microdissected specimens were used, the expression of miR-145 and miR-133a as what was expected. The expression of miR-205 showed similar trends as the cell lines. (RQ, relative quantitation; PUNLMP, papillary urothelial neoplasm of low malignant potential; NIUCLG, non-invasive low-grade urothelial carcinoma; NIUCHG, non-invasive high-grade urothelial carcinoma; INVASIVE, invasive urothelial carcinoma).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNA expression in the “atypical urothelial cells” category of the Paris System for Reporting Urine Cytology. When “atypical urothelial cells” was proven to be benign when followed up (abbreviated as AN), the expression of miR-99a increased as in the “negative” category urine (abbreviated N). When “atypical urothelial cells” was proven malignant when followed up (abbreviated as AU), the expression of miR-99a decreased as in the “malignant” category urine (abbreviated as UC).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing